Next-Generation Sequencing in Conjunction with Digital PCR and Real-time PCR (qPCR)
The integration of multiple molecular biology techniques is key to obtaining comprehensive insights into genomic information. Next-Generation Sequencing (NGS) has revolutionized genomics research, but it is often accompanied by challenges related to its sensitivity and accuracy. By combining NGS with Digital PCR and Real-time PCR (qPCR), researchers can overcome these limitations and obtain more reliable results. They serve as valuable complementary methods to NGS. Prior to sequencing and can be utilized to pre-select specific genomic regions of interest for enrichment, improving the sensitivity of NGS detection. This targeted approach reduces sequencing costs and increases the depth of coverage for the regions of interest.
Digital PCR and Real-time PCR (qPCR) can be employed after NGS to validate and verify the presence of specific genetic variations or mutations identified by the sequencing data. This post-sequencing confirmation enhances the confidence in the detected variants and ensures the accuracy of the results. The combination of NGS offers a comprehensive approach to genomic analysis. This integrated approach improves sensitivity, reduces costs, and enhances the accuracy of genomic data, making it a powerful tool in various research fields, including personalized medicine and disease diagnostics.
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